Human CD10/MME Protein (MME-HM110)
Human CD10/MME Protein (MME-HM110)
Human CD10/MME Protein (MME-HM110)
Human CD10/MME Protein (MME-HM110)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Tyr52-Trp750
Catalog #MME-HM110
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Product Description
 Recombinant Human CD10/MME Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Tyr52-Trp750.
Product Background
 CD10 is an endopeptidase that degrades various bioactive peptides in the extracellular matrix. In addition to enzymatic degradation, it affects multiple intracellular signal transduction pathways. CD10 expression has been extensively studied in human epithelial cancers of numerous organs and sites. CD10 expression pattern depended on the histotypes of thyroid lesions. When possible metastatic tumours and non-epithelial tumours are excluded, high CD10 expression may be useful in determining whether a primary thyroid carcinoma includes an anaplastic component.
Product Category
 Recombinant Protein
Target
 CD10/MME
Synonyms
 Neprilysin; Atriopeptidase; Enkephalinase; NEP; Neutral; SFE; CD10; MME; CALLA; DKFZp686O16152; MGC126681; MGC126707
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Tyr52-Trp750
Molecular Weight
 The protein has a predicted MW of 80.9 kDa. Due to glycosylation, the protein migrates to 81-110 kDa based on Bis-Tris PAGE result.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 50mM MES, 100mM NaCl, 1mM ZnCl2, 10% Glycerol (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD10 is greater than 95% as determined by SEC-HPLC.
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