Name: Human CD117 Protein (CD1-HM117)
Brand: KactusBio
SKU: CD1-HM117-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.73ug/ml determined by ELISA.
Amino Acid Range: Gln26-Thr516

Catalog #CD1-HM117

Size

100UG

500UG

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Human CD117 Protein (CD1-HM117) Summary

Product Description	Recombinant Human CD117 Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Gln26-Thr516.
Product Background	The stem cell factor receptor (SCFR, CD117) is encoded by the c-kit proto-oncogene, which is the cellular counterpart of the v-kit oncogene of a transforming feline retrovirus (HZ4-feline sarcoma virus). It is a 145kD transmembrane glycoprotein which is homologous to several other growth factor receptors such as the receptors for macrophage colony stimulating factor (M-CSF, CSF-1, c-fms), platelet-derived growth factor a and b (PDGF-Ra , PDGF-Rb ), and the newly described growth factor receptor STK-1, which is the human homologue of the murine FLK2/FLT3 receptor.
Product Category	Recombinant Protein / Antibody discovery / ADC targets

Human CD117 Protein (CD1-HM117) Specifications/Details

Protein	CD177
Synonyms	SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT
Accession	P10721-2
Species	Human
Biotinylated	no
Amino Acid Range	Gln26-Thr516
Molecular Weight	The protein has a predicted MW of 56.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.73ug/ml determined by ELISA.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.73ug/ml determined by ELISA.
	Immobilized Human CD117, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA.
	Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-CD117 Ab., hFc Tag on ProA-Biosensor can bind Human CD117, His Tag with an affinity constant of 6.08 nM as determined in BLI assay (Gator® Prime).
	The purity of Human CD117 is greater than 95% as determined by SEC-HPLC.