Product Description |
Recombinant Human CD117 Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Gln26-Thr516. |
Product Background |
The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages. |
Product Category |
Recombinant Protein |
Target |
CD177 |
Synonyms |
SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln26-Thr516 |
Molecular Weight |
The protein has a predicted MW of 56.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 3.42ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 3.42ug/ml determined by ELISA (QC Test). |
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Immobilized Human CD117, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA. |
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Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CD117 is greater than 95% as determined by SEC-HPLC. |
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