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Human CD117 Protein, Ultra Low Endotoxin (CD1-HM117-UL) Summary
Product Description |
Recombinant Human CD117 Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Gln26-Thr516. |
Product Background |
The stem cell factor receptor (SCFR, CD117) is encoded by the c-kit proto-oncogene, which is the cellular counterpart of the v-kit oncogene of a transforming feline retrovirus (HZ4-feline sarcoma virus). It is a 145kD transmembrane glycoprotein which is homologous to several other growth factor receptors such as the receptors for macrophage colony stimulating factor (M-CSF, CSF-1, c-fms), platelet-derived growth factor a and b (PDGF-Ra , PDGF-Rb ), and the newly described growth factor receptor STK-1, which is the human homologue of the murine FLK2/FLT3 receptor. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human CD117 Protein, Ultra Low Endotoxin (CD1-HM117-UL) Specifications/Details
Protein |
CD177 |
Synonyms |
SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln26-Thr516 |
Molecular Weight |
The protein has a predicted MW of 56.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.73ug/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.73ug/ml determined by ELISA. |
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Immobilized Human CD117, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA. |
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Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-CD117 Ab., hFc Tag on ProA-Biosensor can bind Human CD117, His Tag with an affinity constant of 6.08 nM as determined in BLI assay . |
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The purity of Human CD117 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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