Human CD14 Protein (CD1-HM114)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Thr20-Met344

Catalog: CD1-HM114

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Product Description Recombinant Human CD14 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Met344.

Product Background

Human monocyte differentiation antigen CD14 is a pattern recognition receptor (PRR) that enhances innate immune responses. CD14 was first identified as a marker of monocytes to signal intracellular responses upon bacterial encounters. Given the absence of an intracellular tail, CD14 was doubted to have the signaling capacities.

Product Category

Recombinant Protein

Target

CD14
Synonyms CD14; CD14 molecule

Gene/Protein ID

AAH10507

Species

Human

Exact Sequence

Thr20-Met344
Molecular Weight The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Tris-Bis PAGE result.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human CD14 is greater than 95% as determined by SEC-HPLC.
Human CD14 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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