Product Description |
Recombinant Human CD14 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Met344. |
Product Background |
Human monocyte differentiation antigen CD14 is a pattern recognition receptor (PRR) that enhances innate immune responses. CD14 was first identified as a marker of monocytes to signal intracellular responses upon bacterial encounters. Given the absence of an intracellular tail, CD14 was doubted to have the signaling capacities. |
Product Category |
Recombinant Protein |
Target |
CD14 |
Synonyms |
CD14; CD14 molecule |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr20-Met344 |
Molecular Weight |
The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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