Human CD14 Protein (CD1-HM214)
Catalog: CD1-HM214
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| Product Description | Recombinant Human CD14 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Thr20-Met344. |
Product Background |
Human monocyte differentiation antigen CD14 is a pattern recognition receptor (PRR) that enhances innate immune responses. CD14 was first identified as a marker of monocytes to signal intracellular responses upon bacterial encounters. Given the absence of an intracellular tail, CD14 was doubted to have the signaling capacities. |
Product Category |
Recombinant Protein |
Target |
CD14 |
| Accession | |
Species |
Human |
Amino Acid Range |
Thr20-Met344 |
| Molecular Weight | The protein has a predicted MW of 60.99 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-hFc |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Human CD14 is greater than 95% as determined by SEC-HPLC. |
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Human CD14 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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