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Human CD14 Protein (CD1-HM214) Summary
Product Description |
Recombinant Human CD14 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Thr20-Met344. |
Product Background |
Human monocyte differentiation antigen CD14 is a pattern recognition receptor (PRR) that enhances innate immune responses. CD14 was first identified as a marker of monocytes to signal intracellular responses upon bacterial encounters. Given the absence of an intracellular tail, CD14 was doubted to have the signaling capacities. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human CD14 Protein (CD1-HM214) Specifications/Details
Protein |
CD14 |
Synonyms |
CD14; CD14 molecule |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr20-Met344 |
Molecular Weight |
The protein has a predicted MW of 60.99 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human CD14 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD14 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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