• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Thr20-Met344
Catalog #CD1-HM114-UL
Price
Regular price $36300
Regular price Sale price $36300
/
Size
Avantor VWR Fisher Scientific

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Human CD14 Protein, Ultra Low Endotoxin (CD1-HM114-UL) Summary

Product Description

 Recombinant Human CD14 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Met344.

Product Background

 Human monocyte differentiation antigen CD14 is a pattern recognition receptor (PRR) that enhances innate immune responses. CD14 was first identified as a marker of monocytes to signal intracellular responses upon bacterial encounters. Given the absence of an intracellular tail, CD14 was doubted to have the signaling capacities.

Human CD14 Protein, Ultra Low Endotoxin (CD1-HM114-UL) Specifications/Details

Protein
 CD14
Synonyms
 CD14; CD14 molecule
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Thr20-Met344
Molecular Weight
 The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human CD14 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD14 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD14 is greater than 95% as determined by SEC-HPLC. The purity of Human CD14 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...