Human CD155/PVR Protein (CD1-HM255)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.1ug/ml determined by ELISA (QC Test). The affinity constant of 0.958 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Trp21-Asn343

Catalog: CD1-HM255

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Product Description Recombinant Human CD155/PVR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Trp21-Asn343.

Product Background

CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.

Product Category

Recombinant Protein

Target

CD155/PVR
Synonyms CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
Accession

Species

Human

Amino Acid Range

Trp21-Asn343
Molecular Weight The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.1ug/ml determined by ELISA (QC Test). The affinity constant of 0.958 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 uM as determined in SPR assay (Biacore T200).
Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.1ug/ml determined by ELISA (QC Test).
Immobilized Human CD96, mFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA.
Human CD155 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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