Human CD155/PVR Protein (CD1-HM255)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
  • Activity: Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.1ug/ml determined by ELISA (QC Test). The affinity constant of 0.958 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Trp21-Asn343

Catalog: CD1-HM255

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Product Description Recombinant Human CD155/PVR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Trp21-Asn343.
Product Category Recombinant Protein
Target CD155/PVR
Synonyms CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
Gene/Protein ID P15151-1
Species Human
Exact Sequence Trp21-Asn343
Molecular Weight The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.1ug/ml determined by ELISA (QC Test). The affinity constant of 0.958 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 uM as determined in SPR assay (Biacore T200).
Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.1ug/ml determined by ELISA (QC Test).
Immobilized Human CD96, mFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA.
The purity of Human CD155 is greater than 95% as determined by SEC-HPLC.
Human CD155 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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