ELISA curve showing binding between Human TIGIT (hFc tag) and Human CD155 (His tag). OD450 increases with TIGIT concentration, confirming interaction.
Non-biotinylated Human CD155/PVR Protein (CD1-HM455)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 70.5ng/ml determined by ELISA.
  • Amino Acid Range: Trp21-Asn343
Catalog #CD1-HM455
Regular price $264.00
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Size
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Non-biotinylated Human CD155/PVR Protein (CD1-HM455) Summary

Product Description

Recombinant Human CD155/PVR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp21-Asn343.

Product Background

CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Non-biotinylated Human CD155/PVR Protein (CD1-HM455) Specifications/Details

Protein
CD155/PVR
Synonyms
CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Trp21-Asn343
Molecular Weight
The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 70.5ng/ml determined by ELISA.
Product Tag
C-His-Avi

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 70.5ng/ml determined by ELISA. Immobilized Human CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 70.5ng/ml determined by ELISA.
Human CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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