Human CD160 Protein (CD1-HM260)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly25-Leu158

Catalog: CD1-HM260

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Product Description	Recombinant Human CD160 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly25-Leu158.
Product Background	CD160 (also Natural killer cell receptor BY55) is a 27 ‑ 30 kDa member of the Ig superfamily. In human, it is expressed principally on nonmyeloid hematopoietic cells. CD160 antigenis a receptor on immune cells capable to deliver stimulatory or inhibitory signals that regulate cell activation and differentiation. Exists as a GPI-anchored and as a transmembrane form, each likely initiating distinct signaling pathways via phosphoinositol 3-kinase in activated NK cells and via LCK and CD247/CD3 zeta chain in activated T cells.
Product Category	Recombinant Protein

Target	CD160
Synonyms	CD160;-BY55; BY55FLJ46513; NK1; NK28
Gene/Protein ID	O95971-1
Species	Human
Exact Sequence	Gly25-Leu158
Molecular Weight	The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA.
	The purity of Human CD160 is greater than 95% as determined by SEC-HPLC.
	Human CD160 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.