Human CD161 Protein (CD1-HM161)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD161, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. The affinity constant of 78.8 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln67-Ser225

Catalog: CD1-HM161

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Product Description Recombinant Human CD161 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln67-Ser225.
Product Category Recombinant Protein
Target CD161
Synonyms KLRB1; CLEC5B; NKRP1A; NKR-P1A; HNKR-P1a; CD161; Ly59; NKR; NKRP1; NKR-P1; NKRP1ANKR
Gene/Protein ID Q12918-1
Species Human
Exact Sequence Gln67-Ser225
Molecular Weight The protein has a predicted MW of 19.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD161, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. The affinity constant of 78.8 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-CD161 Antibody, hFc tag captured on CM5 Chip via Anti-hFc IgG can bind Human CD161, His Tag with an affinity constant of 78.8 nM as determined in SPR assay (Biacore T200).
Immobilized Human CD161, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
The purity of Human CD161 is greater than 95% as determined by SEC-HPLC.
Human CD161 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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