Name: Human CD161 Protein (CD1-HM161)
Brand: KactusBio
SKU: CD1-HM161-100UG
Price: 379.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD161, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). The affinity constant of 78.8 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gln67-Ser225

Catalog #CD1-HM161

Size

100UG

500UG

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Human CD161 Protein (CD1-HM161) Summary

Product Description	Recombinant Human CD161 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln67-Ser225.
Product Background	CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Human CD161 Protein (CD1-HM161) Specifications/Details

Protein	CD161
Synonyms	KLRB1; CLEC5B; NKRP1A; NKR-P1A; HNKR-P1a; CD161; Ly59; NKR; NKRP1; NKR-P1; NKRP1ANKR
Accession	Q12918-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln67-Ser225
Molecular Weight	The protein has a predicted MW of 19.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD161, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). The affinity constant of 78.8 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-CD161 Antibody, hFc tag captured on CM5 Chip via Anti-hFc IgG can bind Human CD161, His Tag with an affinity constant of 78.8 nM as determined in SPR assay (Biacore T200).
	Immobilized Human CD161, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
	Human CD161 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-CD161 Antibody, hFc Tag on ProA-Biosensor can bind Human CD161, His Tag with an affinity constant of 0.10 µM as determined in BLI assay (Gator® Prime).
	The purity of Human CD161 is greater than 95% as determined by SEC-HPLC.

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