Human CD161 Protein (CD1-HM161)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD161, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA (QC Test). The affinity constant of 78.8 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln67-Ser225

Catalog: CD1-HM161

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Product Description	Recombinant Human CD161 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln67-Ser225.
Product Background	CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment.
Product Category	Recombinant Protein

Target	CD161
Synonyms	KLRB1; CLEC5B; NKRP1A; NKR-P1A; HNKR-P1a; CD161; Ly59; NKR; NKRP1; NKR-P1; NKRP1ANKR
Gene/Protein ID	Q12918-1
Species	Human
Exact Sequence	Gln67-Ser225
Molecular Weight	The protein has a predicted MW of 19.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD161, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA (QC Test). The affinity constant of 78.8 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Anti-CD161 Antibody, hFc tag captured on CM5 Chip via Anti-hFc IgG can bind Human CD161, His Tag with an affinity constant of 78.8 nM as determined in SPR assay (Biacore T200).
	Immobilized Human CD161, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA (QC Test).
	The purity of Human CD161 is greater than 95% as determined by SEC-HPLC.
	Human CD161 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.