Human CD19 Protein, Ultra Low Endotoxin (CD1-HM119-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.50 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Pro20-Lys291
Catalog #CD1-HM119-UL
/
Size
image image

Order by 4pm ET for delivery on:

Human CD19 Protein, Ultra Low Endotoxin (CD1-HM119-UL) Summary

Product Description

 Recombinant Human CD19 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Pro20-Lys291.

Product Background

 CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.

Product Category

 Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets

Human CD19 Protein, Ultra Low Endotoxin (CD1-HM119-UL) Specifications/Details

Protein
 CD19
Synonyms
 CD19; B4; CVID3; Leu-12; MGC12802
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro20-Lys291
Molecular Weight
 The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.50 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human CD19, His Tag with an affinity constant of 0.50 nM as determined in SPR assay (Biacore T200). Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human CD19, His Tag with an affinity constant of 0.50 nM as determined in SPR assay (Biacore T200).
Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.3 ng/ml determined by ELISA (QC Test). Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.3 ng/ml determined by ELISA (QC Test).
Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 11.1 ng/ml determined by ELISA (QC Test). Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 11.1 ng/ml determined by ELISA (QC Test).
Immobilized Human CD19, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 8.5 ng/ml determined by ELISA. Immobilized Human CD19, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 8.5 ng/ml determined by ELISA.
Immobilized Human CD19, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 8.6 ng/ml determined by ELISA. Immobilized Human CD19, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 8.6 ng/ml determined by ELISA.
Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Coltuximab on ProA-Biosensor can bind Human CD19, His Tag with an affinity constant of 5.65 nM as determined in BLI assay . Loaded Coltuximab on ProA-Biosensor can bind Human CD19, His Tag with an affinity constant of 5.65 nM as determined in BLI assay .
Loaded FMC63 on MFc-Biosensor can bind Human CD19, His Tag with an affinity constant of 1.44 nM as determined in BLI assay . Loaded FMC63 on MFc-Biosensor can bind Human CD19, His Tag with an affinity constant of 1.44 nM as determined in BLI assay .
Loaded Inebilizumab on ProA-Biosensor can bind Human CD19, His Tag with an affinity constant of 8.03 nM as determined in BLI assay . Loaded Inebilizumab on ProA-Biosensor can bind Human CD19, His Tag with an affinity constant of 8.03 nM as determined in BLI assay .
Loaded Denintuzumab, hFc Tag on ProA-Biosensor, can bind Human CD19, His Tag with an affinity constant of 3.73 nM as determined in BLI assay (Gator). Loaded Denintuzumab, hFc Tag on ProA-Biosensor, can bind Human CD19, His Tag with an affinity constant of 3.73 nM as determined in BLI assay (Gator).
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recommended Products

Loading...
Recently viewed