• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD1A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD1A Antibody, mFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
  • Amino Acid Range: Asp19-Val300
Catalog #CDA-HM11A
Price
Regular price $390.00
Regular price Sale price $390.00
/
Size
Avantor VWR Fisher Scientific

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Human CD1A Protein (CDA-HM11A) Summary

Product Description

 Recombinant Human CD1A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp19-Val300.

Product Background

 CD1 proteins are a family of major histocompatibility complex (MHC) class I-like antigen-presenting molecules that present lipids to T cells. The cytoplasmic tails (CTs) of all human CD1 isoforms, with the exception of CD1a, contain tyrosine-based sorting motifs, responsible for the internalization of proteins by the clathrin-mediated pathway. CD1a closer to MHC class I in its trafficking and potential antigen-loading compartments among CD1 isoforms.

Human CD1A Protein (CDA-HM11A) Specifications/Details

Protein
 CD1A
Synonyms
 CD1a; CD1A; CD1; FCB6; HTA1; R4; T6
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp19-Val300
Molecular Weight
 The protein has a predicted MW of 33.3 kDa. Due to glycosylation, the protein migrates to 48-53 kDa and 13-14 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD1A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD1A Antibody, mFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD1A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD1A Antibody, mFc Tag with the EC50 of 3.0ng/ml determined by ELISA. Immobilized Human CD1A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD1A Antibody, mFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
Human CD1A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD1A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...