Product Description |
Recombinant Human CD2/SRBC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys25-Asp209. |
Product Background |
The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells. |
Product Category |
Recombinant Protein |
Target |
CD2/SRBC |
Synonyms |
T11; CD2; SRBC; LFA-2; LFA-3 receptor |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys25-Asp209 |
Molecular Weight |
The protein has a predicted MW of 22.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD58, hFc Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test). The affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human CD2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD58, hFc Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test). |
|
|
Human CD2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD2 is greater than 95% as determined by SEC-HPLC. |
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