Human CD2/SRBC Protein (CD2-HM102)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Activity: The affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Lys25-Asp209

Catalog: CD2-HM102

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Product Description	Recombinant Human CD2/SRBC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys25-Asp209.
Product Background	The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells.
Product Category	Recombinant Protein

Target	CD2/SRBC
Synonyms	T11; CD2; SRBC; LFA-2; LFA-3 receptor
Gene/Protein ID	P06729
Species	Human
Exact Sequence	Lys25-Asp209
Molecular Weight	The protein has a predicted MW of 22.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).
	The purity of Human CD2 is greater than 95% as determined by SEC-HPLC.
	Human CD2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.