Human CD200/OX-2 Protein (CD2-HM220)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD200 R1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln31-Gly232

Catalog: CD2-HM220

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Product Description Recombinant Human CD200/OX-2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln31-Gly232.

Product Background

CD200 and its receptors are highly expressed in the lung, on epithelial cells and leukocytes, and emerging evidence links dysregulation of the CD200 pathway with asthma. Moreover, pharmacological modulation of CD200 receptors was shown to improve clinical and inflammatory outcomes of preclinical asthma models.

Product Category

Recombinant Protein

Target

CD200/OX-2
Synonyms CD200; MOX1; MOX2; My033; MRC; OX-2; OX2

Gene/Protein ID

P41217-1

Species

Human

Exact Sequence

Gln31-Gly232
Molecular Weight The protein has a predicted MW of 49.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CD200 R1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD200 R1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
The purity of Human CD200 is greater than 95% as determined by SEC-HPLC.
Human CD200 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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