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Human CD200/OX-2 Protein, Ultra Low Endotoxin (CD2-HM220-UL) Summary
Product Description |
Recombinant Human CD200/OX-2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln31-Gly232. |
Product Background |
CD200 and its receptors are highly expressed in the lung, on epithelial cells and leukocytes, and emerging evidence links dysregulation of the CD200 pathway with asthma. Moreover, pharmacological modulation of CD200 receptors was shown to improve clinical and inflammatory outcomes of preclinical asthma models. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human CD200/OX-2 Protein, Ultra Low Endotoxin (CD2-HM220-UL) Specifications/Details
Protein |
CD200/OX-2 |
Synonyms |
CD200; MOX1; MOX2; My033; MRC; OX-2; OX2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln31-Gly232 |
Molecular Weight |
The protein has a predicted MW of 49.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD200 R1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human CD200 R1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). |
|
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Human CD200 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human CD200 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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