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Human CD228/MFI2 Domain Protein, Ultra Low Endotoxin (MFI-HM12D-UL) Summary
Product Description |
Recombinant Human CD228/MFI2 Domain Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asp358-Cys709. |
Product Background |
Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human CD228/MFI2 Domain Protein, Ultra Low Endotoxin (MFI-HM12D-UL) Specifications/Details
Protein |
CD228/MFI2 |
Synonyms |
Melanotransferrin; CD228; MFI2; MELTF; MAP97; MFI2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp358-Cys709 |
Molecular Weight |
The protein has a predicted MW of 40.49 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD228 Domain, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CD228 Domain, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). |
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Human CD228 Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-CD228 Antibody, hFc Tag on ProA-Biosensor can bind Human CD228 Domain, His Tag with an affinity constant of 1.12 nM as determined in BLI assay. |
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The purity of Human CD228 Domain is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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