Human CD228/MFI2 Protein (MFI-HM128)
Catalog: MFI-HM128
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| Product Description | Recombinant Human CD228/MFI2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys709. |
Product Background |
Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition. |
Product Category |
Recombinant Protein |
Target |
CD228/MFI2 |
Gene/Protein ID |
P08582-1 |
Species |
Human |
Exact Sequence |
Gly20-Cys709 |
| Molecular Weight | The protein has a predicted MW of 76.60 kDa. Due to glycosylation, the protein migrates to 78-88 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Human CD228 is greater than 95% as determined by SEC-HPLC. |
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Human CD228 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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