Product Description |
Recombinant Human CD228/MFI2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys709. |
Product Background |
Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition. |
Product Category |
Recombinant Protein |
Target |
CD228/MFI2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly20-Cys709 |
Molecular Weight |
The protein has a predicted MW of 76.60 kDa. Due to glycosylation, the protein migrates to 78-88 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD228, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human CD228, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. |
|
|
Human CD228 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD228 is greater than 95% as determined by SEC-HPLC. |
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