- Express System: HEK293
- Product Tag: C-hFc
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human CD24, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD24 Antibody, hFc Tag with the EC50 of 0.58ug/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Ser27-Gly59
Product Description |
Recombinant Human CD24 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser27-Gly59. |
Product Background |
CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
CD24 |
Synonyms |
CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser27-Gly59 |
Molecular Weight |
The protein has a predicted MW of 29.9 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD24, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD24 Antibody, hFc Tag with the EC50 of 0.58ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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