Human CD27/TNFRSF7 Protein (CD2-HM127)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human CD27, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 37.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr21-Ile192

Catalog: CD2-HM127

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Product Description Recombinant Human CD27/TNFRSF7 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Thr21-Ile192.
Product Category Recombinant Protein
Target CD27/TNFRSF7
Synonyms CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor
Gene/Protein ID P26842
Species Human
Exact Sequence Thr21-Ile192
Molecular Weight The protein has a predicted MW of 18.1 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD27, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 37.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD27, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 37.9ng/ml determined by ELISA.
The purity of Human CD27 is greater than 95% as determined by SEC-HPLC.
Human CD27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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