Human CD27/TNFRSF7 Protein (CD2-HM227) Summary
Product Description |
Recombinant Human CD27/TNFRSF7 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Thr21-Ile192. |
Product Background |
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human CD27/TNFRSF7 Protein (CD2-HM227) Specifications/Details
Protein |
CD27/TNFRSF7 |
Synonyms |
CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr21-Ile192 |
Molecular Weight |
The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD27 Ligand Trimer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 30.1ng/ml determined by ELISA (QC Test). |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CD27 Ligand Trimer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 30.1ng/ml determined by ELISA (QC Test). |
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Human CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CD27 is greater than 95% as determined by SEC-HPLC. |
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