Human CD28H/IGPR-1 Protein (CD8-HM28H)
Human CD28H/IGPR-1 Protein (CD8-HM28H)
Human CD28H/IGPR-1 Protein (CD8-HM28H)
Human CD28H/IGPR-1 Protein (CD8-HM28H)
Human CD28H/IGPR-1 Protein (CD8-HM28H)
Human CD28H/IGPR-1 Protein (CD8-HM28H)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu23-Gly150
Catalog #CD8-HM28H
Regular price $346.50
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Product Description

Recombinant Human CD28H/IGPR-1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu23-Gly150.

Product Background

CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

CD28H/IGPR-1

Synonyms

TMIGD2; IGPR1; IGPR-1; CD28H; TPC1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Leu23-Gly150

Molecular Weight

The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Cynomolgus B7-H7, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). Cynomolgus B7-H7, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay (Biacore T200).
Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test). Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test).
Human CD28H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD28H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD28H is greater than 95% as determined by SEC-HPLC. The purity of Human CD28H is greater than 95% as determined by SEC-HPLC.

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