Name: Human CD28H/IGPR-1 Protein (CD8-HM28H)
Brand: KactusBio
SKU: CD8-HM28H-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu23-Gly150

Catalog #CD8-HM28H

Size

100UG

500UG

Order now, ships by:

Product Description	Recombinant Human CD28H/IGPR-1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu23-Gly150.
Product Background	CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD28H/IGPR-1
Synonyms	TMIGD2; IGPR1; IGPR-1; CD28H; TPC1
Accession	Q96BF3-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu23-Gly150
Molecular Weight	The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Cynomolgus B7-H7, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay (Biacore T200).
	Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test).
	Human CD28H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD28H is greater than 95% as determined by SEC-HPLC.