Regular price
$346.50
Order by 4pm ET for delivery on:
Human CD28H/IGPR-1 Protein, Ultra Low Endotoxin (CD8-HM28H-UL) Summary
Product Description |
Recombinant Human CD28H/IGPR-1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu23-Gly150. |
Product Background |
CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CD28H/IGPR-1 Protein, Ultra Low Endotoxin (CD8-HM28H-UL) Specifications/Details
Protein |
CD28H/IGPR-1 |
Synonyms |
TMIGD2; IGPR1; IGPR-1; CD28H; TPC1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu23-Gly150 |
Molecular Weight |
The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Cynomolgus B7-H7, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human B7-H7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test). |
|
|
Human CD28H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Loaded Biotinylated Human B7-H7, His-Avi Tag on Streptavidin-Biosensor can bind Human CD28H, hFc Tag with an affinity constant of 5.28 nM as determined in BLI assay . |
|
|
The purity of Human CD28H is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
