• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Thr20-Ser156
Catalog #CD3-HM2LF
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Human CD300LF Protein (CD3-HM2LF) Summary

Product Description

 Recombinant Human CD300LF Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Thr20-Ser156.

Product Background

 Murine norovirus (MNoV) is an important model of human norovirus (HNoV) and mucosal virus infection more broadly. CD300lf as a proteinaceous receptor for MNoV. Interestingly, its paralogue CD300ld was also sufficient for MNoV infection in vitro. CD300lf is essential for both oral and parenteral MNoV infection and to elicit anti-MNoV humoral responses in vivo. human CD300lf (huCD300lf) is not essential for HNoV infection, nor does huCD300lf inhibit binding of HNoV virus-like particles to glycans.

Human CD300LF Protein (CD3-HM2LF) Specifications/Details

Protein
 CD300LF
Synonyms
 CLM-1; IREM-1; IgSF13; CD300F; IGSF13; NKIR; CD300f; CD300LF; CLIM1; DIgR2; LMIR3; NKIR; Pigr3;CLM1; IREM1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Thr20-Ser156
Molecular Weight
 The protein has a predicted MW of 42.1 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human CD300LF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD300LF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD300LF is greater than 95% as determined by SEC-HPLC. The purity of Human CD300LF is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...