Product Description |
Recombinant Human CD300LG/Nepmucin Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu19-Arg247. |
Product Background |
CD300LG is a novel O-glycosylated member of the CD300 antigen-like family. Besides a classical mucin-like domain, it contains a V-type Ig domain. CD300LG binds lymphocyte L-selectin via its Ig domain and supports lymphocyte rolling via its mucin-like domain. The unique structure and function of CD300LG suggest it may play an important role in inflammation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
CD300LG |
Synonyms |
CD300LG; Nepmucin; CLM-9; TREM-4; CD300g; CLM9; TREM4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu19-Arg247 |
Molecular Weight |
The protein has a predicted MW of 26.35 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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