• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Leu19-Arg247
Catalog #CD3-HM1LG
Price
Regular price $22330
Regular price Sale price $22330
/
Size
Avantor VWR Fisher Scientific

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Human CD300LG/Nepmucin Protein (CD3-HM1LG) Summary

Product Description

 Recombinant Human CD300LG/Nepmucin Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu19-Arg247.

Product Background

 CD300LG is a novel O-glycosylated member of the CD300 antigen-like family. Besides a classical mucin-like domain, it contains a V-type Ig domain. CD300LG binds lymphocyte L-selectin via its Ig domain and supports lymphocyte rolling via its mucin-like domain. The unique structure and function of CD300LG suggest it may play an important role in inflammation.

Human CD300LG/Nepmucin Protein (CD3-HM1LG) Specifications/Details

Protein
 CD300LG
Synonyms
 CD300LG; Nepmucin; CLM-9; TREM-4; CD300g; CLM9; TREM4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu19-Arg247
Molecular Weight
 The protein has a predicted MW of 26.35 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human CD300LG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD300LG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD300LG is greater than 95% as determined by SEC-HPLC. The purity of Human CD300LG is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...