Human CD34 Protein (CD3-HM134)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD34, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 19.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser32-Thr290

Catalog: CD3-HM134

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Product Description	Recombinant Human CD34 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser32-Thr290.
Product Background	CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical and clinical associations, CD34 expression is almost ubiquitously related to hematopoietic cells, and it is a common misconception that CD34-positive (CD34()) cells in nonhematopoietic samples represent hematopoietic contamination.
Product Category	Recombinant Protein

Target	CD34
Synonyms	CD34; RP11-328D5.2; CD34 molecule; HPCA1
Gene/Protein ID	P28906-1
Species	Human
Exact Sequence	Ser32-Thr290
Molecular Weight	The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD34, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 19.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD34, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 19.9ng/ml determined by ELISA.
	The purity of Human CD34 is greater than 95% as determined by SEC-HPLC.
	Human CD34 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.