ELISA binding curve showing the interaction between immobilized human CD34 protein and anti-CD34 antibody, with absorbance (OD450) increasing in a dose-dependent manner and an EC50 of 6.9 ng/mL.
Human CD34 Protein (CD3-HM134)
Human CD34 Protein (CD3-HM134)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD34, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
  • Amino Acid Range: Ser32-Thr290
  • Other Available Proteins:
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Catalog #CD3-HM134
Regular price $363.00
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Human CD34 Protein (CD3-HM134) Summary

Product Description

Recombinant Human CD34 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser32-Thr290.

Product Background

CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical and clinical associations, CD34 expression is almost ubiquitously related to hematopoietic cells, and it is a common misconception that CD34-positive (CD34()) cells in nonhematopoietic samples represent hematopoietic contamination.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human CD34 Protein (CD3-HM134) Specifications/Details

Protein
CD34
Synonyms
CD34; RP11-328D5.2; CD34 molecule; HPCA1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser32-Thr290
Molecular Weight
The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD34, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD34, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. Immobilized Human CD34, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
Human CD34 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD34 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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