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Human CD34 Protein, Ultra Low Endotoxin (CD3-HM134-UL) Summary
Product Description |
Recombinant Human CD34 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser32-Thr290. |
Product Background |
CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical and clinical associations, CD34 expression is almost ubiquitously related to hematopoietic cells, and it is a common misconception that CD34-positive (CD34()) cells in nonhematopoietic samples represent hematopoietic contamination. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CD34 Protein, Ultra Low Endotoxin (CD3-HM134-UL) Specifications/Details
Protein |
CD34 |
Synonyms |
CD34; RP11-328D5.2; CD34 molecule; HPCA1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser32-Thr290 |
Molecular Weight |
The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD34, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CD34, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. |
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Human CD34 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CD34 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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