Human CD3D/CD3 delta Protein (CDD-HM101) Summary
Product Description |
Recombinant Human CD3D/CD3 delta Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe22-Ala105. |
Product Background |
T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein.In immunology, the CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human CD3D/CD3 delta Protein (CDD-HM101) Specifications/Details
Protein |
CD3D/CD3 delta |
Synonyms |
CD3d; CD3D; CD3-DELTA; T3D; CD3 delta; T3DOKT3; CD3-DELTA; IMD19 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe22-Ala105 |
Molecular Weight |
The protein has a predicted MW of 10.4 kDa. Due to glycosylation, the protein migrates to 15 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human CD3 delta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human CD3 delta is greater than 95% as determined by SEC-HPLC. |
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