Human CD3E/CD3 epsilon 1-27 peptide Protein (CD3-HM2ED)

  • Express System: Expi293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD3E 1-27 peptide, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp23-Thr48

Catalog: CD3-HM2ED

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Product Description Recombinant Human CD3E/CD3 epsilon 1-27 peptide Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal.It contains Asp23-Thr48.
Product Category Recombinant Protein
Target CD3E/CD3 epsilon 1-27 peptide
Synonyms CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Gene/Protein ID P07766
Species Human
Exact Sequence Asp23-Thr48
Molecular Weight The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD3E 1-27 peptide, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD3E 1-27 peptide, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
The purity of Human CD3E 1-27 peptide is greater than 95% as determined by SEC-HPLC.
Human CD3E 1-27 peptide on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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