Human CD3E/CD3 epsilon 1-27 Protein (CD3-HM2ED)
Human CD3E/CD3 epsilon 1-27 Protein (CD3-HM2ED)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD3E 1-27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp23-Thr48
Catalog #CD3-HM2ED
Regular price $375.00
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Product Description
Recombinant Human CD3E/CD3 epsilon 1-27 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Asp23-Thr48.
Product Background
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Product Category
Recombinant Protein
Target
CD3E/CD3 epsilon 1-27
Synonyms
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Asp23-Thr48
Molecular Weight
The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD3E 1-27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD3E 1-27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA.
Human CD3E 1-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD3E 1-27 is greater than 95% as determined by SEC-HPLC.

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