Human CD3E/CD3 epsilon monomer Protein (CD3-HM20E)
Catalog: CD3-HM20E
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| Product Description | Recombinant Human CD3E/CD3 epsilon monomer Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Asp23-Asp126(C119S,C222S). |
| Product Category | Recombinant Protein |
| Target | CD3E/CD3 epsilon monomer |
| Synonyms | CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
| Gene/Protein ID | P07766 |
| Species | Human |
| Exact Sequence | Asp23-Asp126(C119S,C222S) |
| Molecular Weight | The protein has a predicted MW of 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CD3E at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 26.7ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human CD3E at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 26.7ng/ml determined by ELISA. |
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Immobilized Human CD3E at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 27.7ng/ml / 26ng/ml / 26.7ng/ml determined by ELISA. |
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Human CD3E/CD3 epsilon monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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