Human CD3E/CD3 epsilon Protein (CD3-HM20E)
Catalog: CD3-HM20E
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Product Description | Recombinant Human CD3E/CD3 epsilon Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp23-Asp126(C119S, C122S). |
Product Background |
T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
Product Category |
Recombinant Protein |
Target |
CD3E/CD3 epsilon |
Synonyms | CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
Accession | |
Species |
Human |
Amino Acid Range |
Asp23-Asp126(C119S, C122S) |
Molecular Weight | The protein has a predicted MW of 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human CD3E, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 33.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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