Human CD3E/CD3 epsilon monomer Protein (CD3-HM20E)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human CD3E at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 26.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp23-Asp126(C119S,C222S)

Catalog: CD3-HM20E

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Product Description Recombinant Human CD3E/CD3 epsilon monomer Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Asp23-Asp126(C119S,C222S).
Product Category Recombinant Protein
Target CD3E/CD3 epsilon monomer
Synonyms CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Gene/Protein ID P07766
Species Human
Exact Sequence Asp23-Asp126(C119S,C222S)
Molecular Weight The protein has a predicted MW of 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD3E at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 26.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD3E at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 26.7ng/ml determined by ELISA.
Immobilized Human CD3E at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 27.7ng/ml / 26ng/ml / 26.7ng/ml determined by ELISA.
Human CD3E/CD3 epsilon monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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