Name: Human CD3E/CD3 epsilon Protein (CDE-HM101)
Brand: KactusBio
SKU: CDE-HM101-100UG
Price: 300.0 USD
Availability: InStock

Human CD3E/CD3 epsilon Protein (CDE-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human CD3E, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 9.0 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp23-Asp126

Catalog #CDE-HM101

Size

100UG

500UG

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Product Description	Recombinant Human CD3E/CD3 epsilon Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Asp23-Asp126.
Product Background	CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Product Category	Recombinant Protein

Target	CD3E/CD3 epsilon
Synonyms	CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Accession	P07766
Species	Human
Biotinylated	no
Amino Acid Range	Asp23-Asp126
Molecular Weight	The protein has a predicted MW of 12.6 kDa. Due to glycosylation, the protein migrates to 14-18 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD3E, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 9.0 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD3E, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 9.0 ng/ml determined by ELISA.
	Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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