Regular price
$375.00
Order by 4pm ET for delivery on:
Human CD3E/CD3 epsilon Protein (CDE-HM601) Summary
Product Description |
Recombinant Human CD3E/CD3 epsilon Protein is expressed from HEK293 with Llama Fc tag at the C-terminus.It contains Asp23-Asp126. |
Product Background |
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human CD3E/CD3 epsilon Protein (CDE-HM601) Specifications/Details
Protein |
CD3E/CD3 epsilon |
Synonyms |
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp23-Asp126 |
Molecular Weight |
The protein has a predicted MW of 39.06 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD3E, LIama Fc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA. |
Product Tag |
C-Llama Fc |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human CD3E, LIama Fc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA. |
|
|
Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD3E is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
