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Human CD3E/CD3 epsilon Protein, Ultra Low Endotoxin (CDE-HM101-UL) Summary
Product Description |
Recombinant Human CD3E/CD3 epsilon Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Asp23-Asp126. |
Product Background |
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CD3E/CD3 epsilon Protein, Ultra Low Endotoxin (CDE-HM101-UL) Specifications/Details
Protein |
CD3E/CD3 epsilon |
Synonyms |
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp23-Asp126 |
Molecular Weight |
The protein has a predicted MW of 12.6 kDa. Due to glycosylation, the protein migrates to 14-18 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD3E, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 9.0 ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4). |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CD3E, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 9.0 ng/ml determined by ELISA. |
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Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
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Loaded Anti-CD3 Antibody, hFc Tag on ProA-Biosensor can bind Human CD3E, His Tag with an affinity constant of 2.14 nM as determined in BLI assay . |
Other Available Proteins:
Finding available proteins...
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