Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM157)
Catalog: CD3-HM157
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| Product Description | Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag at the C-terminal.It contains Asp23-Glu120(CD3E)&Gln23-Asn106(CD3G). |
| Product Category | Recombinant Protein |
| Target | CD3E&CD3G/CD3 epsilon&CD3 gamma |
| Synonyms | CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G |
| Gene/Protein ID | P07766(CD3E)&P09693(CD3G) |
| Species | Human |
| Exact Sequence | Asp23-Glu120(CD3E) & Gln23-Asn106(CD3G) |
| Molecular Weight | The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CD3E&CD3G, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2ng/ml determined by ELISA. The affinity constant of 0.22nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human CD3E&CD3G, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.22nM as determined in SPR assay (Biacore T200). |
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Immobilized Human CD3E&CD3G, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2ng/ml determined by ELISA. |
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Immobilized Human CD3E&CD3G, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 36.2ng/ml determined by ELISA. |
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The purity of Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC. |
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Human CD3E&CD3G on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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