Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM157)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)
Catalog #CD3-HM157
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Product Description
 Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Asp126 (CD3E) & Gln23-Ser116 (CD3G).
Product Background
 T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E & CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category
 Recombinant Protein
Target
 CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms
 CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)
Molecular Weight
 The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD3E&CD3G, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2 ng/ml determined by ELISA (QC Test).
Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 36.2 ng/ml determined by ELISA.
Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.

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