Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM157)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD3E&CD3G, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.22nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Asp23-Glu120(CD3E) & Gln23-Asn106(CD3G)

Catalog: CD3-HM157

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Product Description Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu120(CD3E)&Gln23-Asn106(CD3G).

Product Background

T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Product Category

Recombinant Protein

Target

CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G

Gene/Protein ID

P07766(CD3E)&P09693(CD3G)

Species

Human

Exact Sequence

Asp23-Glu120(CD3E) & Gln23-Asn106(CD3G)
Molecular Weight The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CD3E&CD3G, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.22nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD3E&CD3G, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.22nM as determined in SPR assay (Biacore T200).
Immobilized Human CD3E&CD3G, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2ng/ml determined by ELISA (QC Test).
Immobilized Human CD3E&CD3G, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 36.2ng/ml determined by ELISA.
The purity of Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.
Human CD3E&CD3G on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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