Human CD4/LEU3 Protein (CD4-HM204)
Catalog: CD4-HM204
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| Product Description | Recombinant Human CD4/LEU3 Protein is expressed from Expi293 with hFc at the C-terminal.; It contains Lys26-Trp390. |
| Product Category | Recombinant Protein |
| Target | CD4/LEU3 |
| Synonyms | CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3 |
| Gene/Protein ID | P01730 |
| Species | Human |
| Exact Sequence | Lys26-Trp390 |
| Molecular Weight | The protein has a predicted MW of 67.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CD4 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS, 100mM L-Arginine (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human CD4 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA. |
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The purity of Human CD4 is greater than 95% as determined by SEC-HPLC. |
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Human CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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