• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD4 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Lys26-Trp390
Catalog #CD4-HM204
Price
Regular price $21450
Regular price Sale price $21450
/
Size
Avantor VWR Fisher Scientific

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Human CD4/LEU3 Protein (CD4-HM204) Summary

Product Description

 Recombinant Human CD4/LEU3 Protein is expressed from HEK293 with hFc (IgG1) at the C-Terminus.It contains Lys26-Trp390.

Product Background

 CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.

Human CD4/LEU3 Protein (CD4-HM204) Specifications/Details

Protein
 CD4/LEU3
Synonyms
 CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys26-Trp390
Molecular Weight
 The protein has a predicted MW of 67.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD4 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA (QC Test).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD4 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA (QC Test). Immobilized Human CD4 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA (QC Test).
Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD4 is greater than 95% as determined by SEC-HPLC. The purity of Human CD4 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...