Human CD4/LEU3 Protein (CD4-HM204)
Human CD4/LEU3 Protein (CD4-HM204)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 24.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys26-Trp390
Catalog #CD4-HM204
Regular price $195.00
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Product Description
Recombinant Human CD4/LEU3 Protein is expressed from HEK293 with hFc at the C-Terminus.It contains Lys26-Trp390.
Product Background
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Product Category
Recombinant Protein
Target
CD4/LEU3
Synonyms
CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Lys26-Trp390
Molecular Weight
The protein has a predicted MW of 67.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 24.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 24.8ng/ml determined by ELISA.
Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD4 is greater than 95% as determined by SEC-HPLC.

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