Human CD4/LEU3 Protein (CD4-HM204)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 24.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Lys26-Trp390

Catalog: CD4-HM204

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Product Description	Recombinant Human CD4/LEU3 Protein is expressed from HEK293 with hFc at the C-Terminus.It contains Lys26-Trp390.
Product Background	CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Product Category	Recombinant Protein

Target	CD4/LEU3
Synonyms	CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
Gene/Protein ID	P01730
Species	Human
Exact Sequence	Lys26-Trp390
Molecular Weight	The protein has a predicted MW of 67.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 24.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 24.8ng/ml determined by ELISA.
	The purity of Human CD4 is greater than 95% as determined by SEC-HPLC.
	Human CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.