Human CD4/LEU3 Protein (CD4-HM204)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human CD4 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Lys26-Trp390

Catalog: CD4-HM204

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Product Description Recombinant Human CD4/LEU3 Protein is expressed from Expi293 with hFc at the C-terminal.; It contains Lys26-Trp390.
Product Category Recombinant Protein
Target CD4/LEU3
Synonyms CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
Gene/Protein ID P01730
Species Human
Exact Sequence Lys26-Trp390
Molecular Weight The protein has a predicted MW of 67.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD4 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS, 100mM L-Arginine (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD4 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA.
The purity of Human CD4 is greater than 95% as determined by SEC-HPLC.
Human CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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