Human CD4/LEU3 Protein (CD4-HM204)
Human CD4/LEU3 Protein (CD4-HM204)
Human CD4/LEU3 Protein (CD4-HM204)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD4 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Lys26-Trp390
Catalog #CD4-HM204
Regular price $214.50
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Product Description

Recombinant Human CD4/LEU3 Protein is expressed from HEK293 with hFc at the C-Terminus.It contains Lys26-Trp390.

Product Background

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

CD4/LEU3

Synonyms

CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Lys26-Trp390

Molecular Weight

The protein has a predicted MW of 67.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CD4 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD4 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA (QC Test). Immobilized Human CD4 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA (QC Test).
Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD4 is greater than 95% as determined by SEC-HPLC. The purity of Human CD4 is greater than 95% as determined by SEC-HPLC.

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