Product Description |
Recombinant Human CD4/LEU3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys26-Trp390. |
Product Background |
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. |
Product Category |
Recombinant Protein |
Target |
CD4/LEU3 |
Synonyms |
CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys26-Trp390 |
Molecular Weight |
The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 10.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 10.6ng/ml determined by ELISA. |
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Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Immobilized Human CD4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 10.2ng/ml / 10.6ng/ml / 10.6ng/ml determined by ELISA. |
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The purity of Human CD4 is greater than 95% as determined by SEC-HPLC |
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