Human CD4/LEU3 Protein (CD4-HM401)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 10.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Lys26-Trp390

Catalog: CD4-HM401

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Product Description	Recombinant Human CD4/LEU3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys26-Trp390.
Product Background	CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Product Category	Recombinant Protein

Target	CD4/LEU3
Synonyms	CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
Gene/Protein ID	P01730
Species	Human
Exact Sequence	Lys26-Trp390
Molecular Weight	The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 10.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD4 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 10.6ng/ml determined by ELISA.
	Immobilized Human CD4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 10.2ng/ml / 10.6ng/ml / 10.6ng/ml determined by ELISA.
	The purity of Human CD4 is greater than 95% as determined by SEC-HPLC
	Human CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.