• Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD40, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand, His Tag with the EC50 of 57.6ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gln114-Leu261
Catalog #CDL-HE14L
Price
Regular price $33000
Regular price Sale price $33000
/
Size
Avantor VWR Fisher Scientific

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Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Protein (CDL-HE14L) Summary

Product Description

 Recombinant Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Protein is expressed from E.coli with His tag at the C-terminus.It contains Gln114-Leu261.

Product Background

 CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Protein (CDL-HE14L) Specifications/Details

Protein
 CD40 Ligand/TNFSF5
Synonyms
 CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Accession
Species
 Human/Cynomolgus/Rhesus macaque
Biotinylated
 no
Amino Acid Range
 Gln114-Leu261
Molecular Weight
 The protein has a predicted MW of 17.15 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human CD40, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand, His Tag with the EC50 of 57.6ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 10% glycerol (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD40, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand, His Tag with the EC50 of 57.6ng/ml determined by ELISA (QC Test). Immobilized Human CD40, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand, His Tag with the EC50 of 57.6ng/ml determined by ELISA (QC Test).
Human/Cynomolgus/Rhesus macaque CD40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human/Cynomolgus/Rhesus macaque CD40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...