Product Description |
Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Met113-Leu261. |
Product Background |
CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.-. |
Product Category |
Recombinant Protein |
Target |
CD40 Ligand/TNFSF5 Trimer |
Synonyms |
CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met113-Leu261 |
Molecular Weight |
The protein has a predicted MW of 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD40, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-monomeric hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human CD40, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test). |
|
|
Human CD40 Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml. |
|
|
The purity of Human CD40 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
