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Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM240) Summary
Product Description |
Recombinant Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Met113-Leu261 trimer design. |
Product Background |
CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.-. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM240) Specifications/Details
Protein |
CD40 Ligand/TNFSF5 |
Synonyms |
CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
Accession |
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Species |
Human/Cynomolgus/Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Met113-Leu261 |
Molecular Weight |
The protein has a predicted MW of 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD40, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test). |
Product Tag |
N-monomeric hFc |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CD40, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test). |
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Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml. |
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Loaded Human CD40, His Tag on Anti-His-Biosensor can bind Human CD40 Ligand (Trimer), monomeric hFc Tag with an affinity constant of 0.61 nM as determined in BLI assay . |
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The purity of Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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