Human CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM240)

  • Express System: HEK293
  • Product Tag: N-monomeric hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD40, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Met113-Leu261

Catalog: CDL-HM240

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Product Description Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Met113-Leu261.
Product Category Recombinant Protein
Target CD40 Ligand/TNFSF5 Trimer
Synonyms CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Gene/Protein ID P29965
Species Human
Exact Sequence Met113-Leu261
Molecular Weight The protein has a predicted MW of 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD40, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag N-monomeric hFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD40, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.
The purity of Human CD40 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
Human CD40 Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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