Name: Human CD40 Ligand/TNFSF5 Trimer Protein, Ultra Low Endotoxin (CDL-HM140-UL)
Brand: KactusBio
SKU: CDL-HM140-UL-100UG
Price: 327.0 USD
Availability: InStock

Human CD40 Ligand/TNFSF5 Trimer Protein, Ultra Low Endotoxin (CDL-HM140-UL)

Immobilized Human CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA.

Human CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human CD40, hFc Tag on ProA-Biosensor can bind Human CD40 Ligand (Trimer), His-Flag Tag with an affinity constant of 0.23 µM as determined in BLI assay (Gator® Prime).

The purity of Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Met113-Leu261

Catalog #CDL-HM140-UL

Size

100UG

500UG

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Product Description	Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Met113-Leu261 trimer design.
Product Background	CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD40 Ligand/TNFSF5
Synonyms	CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Accession	P29965
Species	Human
Biotinylated	no
Amino Acid Range	Met113-Leu261
Molecular Weight	The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA.
	Human CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human CD40, hFc Tag on ProA-Biosensor can bind Human CD40 Ligand (Trimer), His-Flag Tag with an affinity constant of 0.23 µM as determined in BLI assay (Gator® Prime).
	The purity of Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.