• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD42b, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD42b Antibody, hFc Tag with the EC50 of 21.1ng/ml determined by ELISA.
  • Amino Acid Range: His17-Leu531
Catalog #GP1-HM2BA
Price
Regular price $345.00
Regular price Sale price $345.00
/
Size
Avantor VWR Fisher Scientific

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Human CD42b/GP1BA Protein (GP1-HM2BA) Summary

Product Description

 Recombinant Human CD42b/GP1BA Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains His17-Leu531.

Product Background

 Absence of the CD42 complex leads to the Bernard-Soulier syndrome (BSS), which is a bleeding disorder characterized by thrombocytopenia and giant platelets. So far, mutations have been found in the genes encoding CD42a, CD42b and CD42c in patients with BSS**.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human CD42b/GP1BA Protein (GP1-HM2BA) Specifications/Details

Protein
 CD42b/GP1BA
Synonyms
 GP-Ib alpha; GPIb-alpha; GPIbA; GP1BA; BDPLT1; BDPLT3; BSS; CD42B; CD42b-alpha; DBPLT3; GP1B; VWDP; BP1BA; MGC34595
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 His17-Leu531
Molecular Weight
 The protein has a predicted MW of 82.47 kDa. Due to glycosylation, the protein migrates to 130-170 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD42b, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD42b Antibody, hFc Tag with the EC50 of 21.1ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 100mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD42b, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD42b Antibody, hFc Tag with the EC50 of 21.1ng/ml determined by ELISA. Immobilized Human CD42b, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD42b Antibody, hFc Tag with the EC50 of 21.1ng/ml determined by ELISA.
Human CD42b on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD42b on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD42b is greater than 95% as determined by SEC-HPLC. The purity of Human CD42b is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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