Human CD45/PTPRC Protein (PTP-HM104)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD45, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln26-Lys577

Catalog: PTP-HM104

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Product Description Recombinant Human CD45/PTPRC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Lys577.

Product Background

PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts.

Product Category

Recombinant Protein

Target

CD45/PTPRC
Synonyms L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48
Accession

Species

Human

Amino Acid Range

Gln26-Lys577
Molecular Weight The protein has a predicted MW of 61.9 kDa. Due to glycosylation, the protein migrates to 118-150 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CD45, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD45, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA.
The purity of Human CD45 is greater than 95% as determined by SEC-HPLC.
Human CD45 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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