Human CD45/PTPRC Protein (PTP-HM104)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD45, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln26-Lys577

Catalog: PTP-HM104

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Product Description	Recombinant Human CD45/PTPRC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Lys577.
Product Background	PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts.
Product Category	Recombinant Protein

Target	CD45/PTPRC
Synonyms	L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48
Gene/Protein ID	P08575-3
Species	Human
Exact Sequence	Gln26-Lys577
Molecular Weight	The protein has a predicted MW of 61.9 kDa. Due to glycosylation, the protein migrates to 118-150 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD45, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD45, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA.
	The purity of Human CD45 is greater than 95% as determined by SEC-HPLC.
	Human CD45 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.