Product Description |
Recombinant Human CD45/PTPRC Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln26-Lys577. |
Product Background |
PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts. |
Product Category |
Recombinant Protein |
Protein |
CD45/PTPRC |
Synonyms |
L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln26-Lys577 |
Molecular Weight |
The protein has a predicted MW of 87.53 kDa. Due to glycosylation, the protein migrates to 120-160 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized AHuman CD45, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD45 Antibody, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized AHuman CD45, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD45 Antibody, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA. |
|
|
Human CD45 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD45 is greater than 95% as determined by SEC-HPLC. |
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