Name: Human CD45/PTPRC Protein, Ultra Low Endotoxin (PTP-HM104-UL)
Brand: KactusBio
SKU: PTP-HM104-UL-100UG
Price: 381.0 USD
Availability: InStock

Human CD45/PTPRC Protein, Ultra Low Endotoxin (PTP-HM104-UL)

Immobilized Human CD45, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA (QC Test).

Human CD45 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD45 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD45, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gln26-Lys577

Catalog #PTP-HM104-UL

Size

100UG

500UG

Order now, ships by:

[{"id":47049786654942,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"PTP-HM104-UL-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Human CD45\/PTPRC Protein, Ultra Low Endotoxin (PTP-HM104-UL) - 100UG","public_title":"100UG","options":["100UG"],"price":38100,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":47049904783582,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"PTP-HM104-UL-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Human CD45\/PTPRC Protein, Ultra Low Endotoxin (PTP-HM104-UL) - 500UG","public_title":"500UG","options":["500UG"],"price":127100,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Product Description	Recombinant Human CD45/PTPRC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Lys577.
Product Background	PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD45/PTPRC
Synonyms	L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48
Accession	P08575-3
Species	Human
Biotinylated	no
Amino Acid Range	Gln26-Lys577
Molecular Weight	The protein has a predicted MW of 61.9 kDa. Due to glycosylation, the protein migrates to 118-150 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD45, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD45, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA (QC Test).
	Human CD45 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD45 is greater than 95% as determined by SEC-HPLC.