Product Description |
Recombinant Human CD52 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Gly25-Ser36. |
Product Background |
CD52, also known as CAMPATH-1 antigen, HE5, and gp20, is a cell surface glycoprotein that can be targeted to induce immune suppression by complement-mediated cell lysis. Mature human CD52 is a 12 amino acid peptide that is tethered to the cell surface with a GPI linkage.CD52 may play a role in carrying and orienting carbohydrate, as well as having a more specific role. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
CD52 |
Synonyms |
CD52 molecule; CD52; CDW52 antigen (CAMPATH-1 antigen); CDw52; He5; HEL-S-171mP; CAMPATH-1; CAMPATH1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Ser36 |
Molecular Weight |
The protein has a predicted MW of 27.6 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-mFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human CD52 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
|
|
The purity of-Human CD52 is greater than 95% as determined by SEC-HPLC. |
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