Human CD58 Protein (CD5-HM108)

Immobilized Human CD58, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD2, hFc Tag (Cat. CD2-HM202) with the EC50 of 39.7ng/ml determined by ELISA.

Human CD58 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD58 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD58, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD2, hFc Tag (Cat. CD2-HM202) with the EC50 of 39.7ng/ml determined by ELISA.
Amino Acid Range: Phe29-Arg215

Catalog #CD5-HM108

Price

$240⁰⁰

Size

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Human CD58 Protein (CD5-HM108) Summary

Product Description

Recombinant Human CD58 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Phe29-Arg215.

Product Background

Loss of CD58 is a common mechanism for tumor immune evasion in lymphoid malignancies. CD58 loss is known to occur due to both genetic and non-genetic causes; therefore, we hypothesized that restoring CD58 expression in lymphoma cells may be an effective treatment approach. EZH2 is involved in the epigenetic silencing of CD58 in lymphoma cells as a mechanism for tumor immune escape, and EZH2 inhibitors are able to restore epigenetically suppressed CD58 expression.

Human CD58 Protein (CD5-HM108) Specifications/Details

Protein	CD58
Synonyms	Lymphocyte function-associated antigen 3;Ag3;CD58;LFA3
Accession	AAH05930
Species	Human
Biotinylated	no
Amino Acid Range	Phe29-Arg215
Molecular Weight	The protein has a predicted MW of 23.07 kDa. Due to glycosylation, the protein migrates to 35-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD58, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD2, hFc Tag (Cat. CD2-HM202) with the EC50 of 39.7ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human CD58, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD2, hFc Tag (Cat. CD2-HM202) with the EC50 of 39.7ng/ml determined by ELISA.
	Human CD58 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD58 is greater than 95% as determined by SEC-HPLC.