Name: Human CD58 Protein (CD5-HM208)
Brand: KactusBio
SKU: CD5-HM208-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD58, hFc Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test). The affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Phe29-Arg215

Catalog #CD5-HM208

Size

100UG

500UG

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Human CD58 Protein (CD5-HM208) Summary

Product Description	Recombinant Human CD58 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Phe29-Arg215.
Product Background	Loss of CD58 is a common mechanism for tumor immune evasion in lymphoid malignancies. CD58 loss is known to occur due to both genetic and non-genetic causes; therefore, we hypothesized that restoring CD58 expression in lymphoma cells may be an effective treatment approach. EZH2 is involved in the epigenetic silencing of CD58 in lymphoma cells as a mechanism for tumor immune escape, and EZH2 inhibitors are able to restore epigenetically suppressed CD58 expression.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human CD58 Protein (CD5-HM208) Specifications/Details

Protein	CD58
Synonyms	Lymphocyte function-associated antigen 3;Ag3;CD58;LFA3
Accession	AAH05930
Species	Human
Biotinylated	no
Amino Acid Range	Phe29-Arg215
Molecular Weight	The protein has a predicted MW of 48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD58, hFc Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test). The affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).
	Immobilized Human CD2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD58, hFc Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test).
	Human CD58 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Biotinylated Human CD2, His-Avi Tag (Cat. CD2-HM402B) on Steptavidin-Biosensor can bind Human CD58, hFc Tag with an affinity constant of 5.28 nM as determined in BLI assay.
	The purity of Human CD58 is greater than 95% as determined by SEC-HPLC.

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