Product Description |
Recombinant Human CD58 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe29-Arg215. |
Product Background |
Loss of CD58 is a common mechanism for tumor immune evasion in lymphoid malignancies. CD58 loss is known to occur due to both genetic and non-genetic causes; therefore, we hypothesized that restoring CD58 expression in lymphoma cells may be an effective treatment approach. EZH2 is involved in the epigenetic silencing of CD58 in lymphoma cells as a mechanism for tumor immune escape, and EZH2 inhibitors are able to restore epigenetically suppressed CD58 expression. |
Product Category |
Recombinant Protein |
Target |
CD58 |
Synonyms |
Lymphocyte function-associated antigen 3；Ag3；CD58；LFA3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe29-Arg215 |
Molecular Weight |
The protein has a predicted MW of 48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD58, hFc Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test). The affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human CD2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD58, hFc Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test). |
|
|
Human CD58 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD58 is greater than 95% as determined by SEC-HPLC. |
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