Human CD58 Protein (CD5-HM208)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Phe29-Arg215

Catalog: CD5-HM208

$240.00
Size:

Order now get it on

Product Description Recombinant Human CD58 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe29-Arg215.

Product Background

Loss of CD58 is a common mechanism for tumor immune evasion in lymphoid malignancies. CD58 loss is known to occur due to both genetic and non-genetic causes; therefore, we hypothesized that restoring CD58 expression in lymphoma cells may be an effective treatment approach. EZH2 is involved in the epigenetic silencing of CD58 in lymphoma cells as a mechanism for tumor immune escape, and EZH2 inhibitors are able to restore epigenetically suppressed CD58 expression.

Product Category

Recombinant Protein

Target

CD58
Synonyms Lymphocyte function-associated antigen 3;Ag3;CD58;LFA3

Gene/Protein ID

AAH05930

Species

Human

Exact Sequence

Phe29-Arg215
Molecular Weight The protein has a predicted MW of 48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).
The purity of Human CD58 is greater than 95% as determined by SEC-HPLC.
Human CD58 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in