Human CD58 Protein (CD5-HM208)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Phe29-Arg215

Catalog: CD5-HM208

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Product Description Recombinant Human CD58 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe29-Arg215.
Product Category Recombinant Protein
Target CD58
Synonyms Lymphocyte function-associated antigen 3;Ag3;CD58;LFA3
Gene/Protein ID AAH05930
Species Human
Exact Sequence Phe29-Arg215
Molecular Weight The protein has a predicted MW of 48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).
The purity of Human CD58 is greater than 95% as determined by SEC-HPLC.
Human CD58 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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