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Human CD59 Protein, Ultra Low Endotoxin (CD5-HM259-UL) Summary
Product Description |
Recombinant Human CD59 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu26-Asn102. |
Product Background |
CD59 regulates complement activation cascade at the final step, inhibiting formation of membrane attack complex (MAC). This protein, being anchored to the cell membrane via glycosyl phosphatidyl inositol (GPI), is expressed ubiquitously on cells which are in contact with body fluids containing components. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human CD59 Protein, Ultra Low Endotoxin (CD5-HM259-UL) Specifications/Details
Protein |
CD59 |
Synonyms |
CD59; 1F5; HRF-20; HRF20; MAC-IP; MACIP; MEM43; MACIF; MIRL; G344; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu26-Asn102 |
Molecular Weight |
The protein has a predicted MW of 35.73 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human CD59 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human CD59 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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