• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.53 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: His18-Leu402
Catalog #CD6-HM101
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Human CD6 Protein (CD6-HM101) Summary

Product Description

 Recombinant Human CD6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His18-Leu402.

Product Background

 The cell surface glycoprotein CD6 is expressed on leukocytes and mediates T cell trafficking across endothelial cell barriers. CD6 is a lymphocyte surface co-receptor physically associated with the T-cell receptor (TCR)/CD3 complex at the center of the immunological synapse.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human CD6 Protein (CD6-HM101) Specifications/Details

Protein
 CD6
Synonyms
 CD6; TP120; T12
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 His18-Leu402
Molecular Weight
 The protein has a predicted MW of 42.14 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.53 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 uM as determined in SPR assay (Biacore T200). Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 uM as determined in SPR assay (Biacore T200).
Immobilized Human CD6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). Immobilized Human CD6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).
Human CD6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD6 is greater than 95% as determined by SEC-HPLC. The purity of Human CD6 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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