Name: Human CD6 Protein (CD6-HM101)
Brand: KactusBio
SKU: CD6-HM101-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.53 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: His18-Leu402

Catalog #CD6-HM101

Size

100UG

500UG

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Product Description	Recombinant Human CD6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His18-Leu402.
Product Background	The cell surface glycoprotein CD6 is expressed on leukocytes and mediates T cell trafficking across endothelial cell barriers. CD6 is a lymphocyte surface co-receptor physically associated with the T-cell receptor (TCR)/CD3 complex at the center of the immunological synapse.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD6
Synonyms	CD6; TP120; T12
Accession	NP_006716.3
Species	Human
Biotinylated	no
Amino Acid Range	His18-Leu402
Molecular Weight	The protein has a predicted MW of 42.14 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.53 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 uM as determined in SPR assay (Biacore T200).
	Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
	Human CD6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD6 is greater than 95% as determined by SEC-HPLC.