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Human CD73/NT5E Protein, Ultra Low Endotoxin (CD7-HM273-UL) Summary
Product Description |
Recombinant Human CD73/NT5E Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Trp27-Lys547. |
Product Background |
CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A- deficiency of CD73 occurs in a variety of immunodeficiency diseases. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human CD73/NT5E Protein, Ultra Low Endotoxin (CD7-HM273-UL) Specifications/Details
Protein |
CD73/NT5E |
Synonyms |
CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Trp27-Lys547 |
Molecular Weight |
The protein has a predicted MW of 84.5 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD73, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 35.3ng/ml determined by ELISA (QC Test). See testing image for detail.Measured-by-its-ability-to-hydrolyze-the-5'-phosphate-group-from-the-substrate-adenosine-5'-monophosphate- (AMP). The specific activity is >10000 pmol/min/ug (QC Test). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CD73, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 35.3ng/ml determined by ELISA (QC Test). |
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Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CD73 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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