Human CD74/DHLAG Protein, Ultra Low Endotoxin (CD7-HM175-UL)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD74 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln73-Met232
Catalog #CD7-HM175-UL
Regular price $290.00
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Product Description

Recombinant Human CD74/DHLAG Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln73-Met232.

Product Background

CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

CD74/DHLAG

Synonyms

CD74; Ii; DHLAG; HLADG; Ia-GAMMA; p33; CLIP; INVG34; Ia GAMMA ; II

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gln73-Met232

Molecular Weight

The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CD74 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 0.001 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 24 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD74 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. Immobilized Human CD74 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Human CD74 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD74 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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