Human CD8 alpha&beta Heterodimer Protein (CD8-HM1AB)

  • Express System: HEK293
  • Product Tag: C-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD8A&CD8B, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail

Catalog: CD8-HM1AB

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Product Description Recombinant Human CD8 alpha&beta Heterodimer Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Ser22-Asp182(CD8 alpha) acidic tail and Leu22-Pro170(CD8 beta) basic tail.

Product Background

CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR.

Product Category

Recombinant Protein

Target

CD8 alpha&beta

Gene/Protein ID

P01732-1(CD8 alpha)&P10966-1(CD8 beta)

Species

Human

Exact Sequence

Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail
Molecular Weight The protein has a predicted MW of 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 26-28 kDa (CD8 alpha) and 28-32 kDa (CD8 beta) based on Tris-Bis PAGE result.

Activity

Immobilized Human CD8A&CD8B, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Flag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD8A&CD8B, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA.
The purity of Human CD8 alpha&beta Heterodimer is greater than 95% as determined by SEC-HPLC.
Human CD8 alpha&beta Heterodimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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