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Human CD8 alpha&beta Heterodimer Protein (CD8-HM1AB) Summary
Product Description |
Recombinant Human CD8 alpha&beta Heterodimer Protein is expressed as a heterodimer of CD8 alpha and CD8 beta by co-expression in HEK293. Subunit CD8 alpha is fused with a His tag at the C-terminus and subunit CD8 beta is fused with a Flag tag at the C-terminus.It contains Ser22-Asp182 (CD8 alpha) acidic tail and Leu22-Pro170 (CD8 beta) basic tail. |
Product Background |
CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CD8 alpha&beta Heterodimer Protein (CD8-HM1AB) Specifications/Details
Protein |
CD8 alpha&beta |
Synonyms |
CD8 alphaβ CD8A&CD8B; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail |
Molecular Weight |
The protein has a predicted MW of 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 32-38 kDa and 40-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD8A&CD8B, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. |
Product Tag |
C-His, C-Flag |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CD8A&CD8B, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. |
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Immobilized Human CD8 alpha&beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 alpha&beta Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. |
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Immobilized Human CD8 alpha &beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 beta Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. |
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Human CD8 alpha&beta Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CD8 alpha&beta Heterodimer is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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