Product Description |
Recombinant Human CD8 alpha&beta Heterodimer Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Ser22-Asp182(CD8 alpha) acidic tail and Leu22-Pro170(CD8 beta) basic tail. |
Product Background |
CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR. |
Product Category |
Recombinant Protein |
Target |
CD8 alpha&beta |
Synonyms |
CD8 alphaβ CD8A&CD8B; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail |
Molecular Weight |
The protein has a predicted MW of 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 26-28 kDa (CD8 alpha) and 28-32 kDa (CD8 beta) based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD8A&CD8B, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Flag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human CD8A&CD8B, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test). |
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Human CD8 alpha&beta Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CD8 alpha&beta Heterodimer is greater than 95% as determined by SEC-HPLC. |
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