Human CD8 alpha&beta Heterodimer Protein (CD8-HM1AB)
Catalog: CD8-HM1AB
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| Product Description | Recombinant Human CD8 alpha&beta Heterodimer Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Ser22-Asp182(CD8 alpha) acidic tail and Leu22-Pro170(CD8 beta) basic tail. |
Product Background |
CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR. |
Product Category |
Recombinant Protein |
Target |
CD8 alpha&beta |
| Accession | |
Species |
Human |
Amino Acid Range |
Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail |
| Molecular Weight | The protein has a predicted MW of 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 26-28 kDa (CD8 alpha) and 28-32 kDa (CD8 beta) based on Tris-Bis PAGE result. |
Activity |
Immobilized Human CD8A&CD8B, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Flag |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human CD8A&CD8B, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. |
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The purity of Human CD8 alpha&beta Heterodimer is greater than 95% as determined by SEC-HPLC. |
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Human CD8 alpha&beta Heterodimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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