Name: Human CD8 alpha&beta Heterodimer Protein (CD8-HM1AB)
Brand: KactusBio
SKU: CD8-HM1AB-100UG
Price: 379.5 USD
Availability: InStock

ELISA binding curve showing dose-dependent response of anti-CD8 antibody to recombinant human CD8A&CD8B protein with His tag

Human CD8 alpha&beta Heterodimer Protein (CD8-HM1AB)

Immobilized Human CD8A&CD8B, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test).

Human CD8 alpha&beta Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD8 alpha&beta Heterodimer is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His, C-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human CD8A&CD8B, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test).
Amino Acid Range: Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail

Catalog #CD8-HM1AB

Size

100UG

500UG

Order by 4pm ET for delivery on:

[{"id":44284842606814,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"CD8-HM1AB-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Human CD8 alpha\u0026beta Heterodimer Protein (CD8-HM1AB) - 100UG","public_title":"100UG","options":["100UG"],"price":37950,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":44284860661982,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"CD8-HM1AB-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Human CD8 alpha\u0026beta Heterodimer Protein (CD8-HM1AB) - 500UG","public_title":"500UG","options":["500UG"],"price":126500,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Human CD8 alpha&beta Heterodimer Protein (CD8-HM1AB) Summary

Product Description	Recombinant Human CD8 alpha&beta Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus of CD8 alpha and Flag tag at the C-Terminus of CD8 beta.It contains Ser22-Asp182(CD8 alpha) acidic tail and Leu22-Pro170(CD8 beta) basic tail.
Product Background	CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8⁺ peripheral T cells bearing TCR.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human CD8 alpha&beta Heterodimer Protein (CD8-HM1AB) Specifications/Details

Protein	CD8 alpha&beta
Synonyms	CD8 alphaβ CD8A&CD8B;
Accession	P01732-1(CD8 alpha)&P10966-1(CD8 beta)
Species	Human
Biotinylated	no
Amino Acid Range	Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail
Molecular Weight	The protein has a predicted MW of 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD8A&CD8B, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test).
Product Tag	C-His, C-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human CD8A&CD8B, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test).
	Human CD8 alpha&beta Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD8 alpha&beta Heterodimer is greater than 90% as determined by SEC-HPLC.